Recombinant mouse IgG2a isotype control

Recombinant mouse IgG2a isotype control mAb against E. coli β-galactosidase

InvivoGen’s Anti-β-Gal-mIgG2a InvivoFit™ antibody

InvivoGen provides a recombinant anti-β-Gal-mIgG2a monoclonal antibody (mAb). It is expressed and produced in chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided. This control antibody targets E. coli's β-galactosidase (β-Gal), an antigen that is not present in mice. In addition, it displays very high ADCP (antibody-dependent cell-mediated phagocytosis), high ADCC (antibody-dependent cell-mediated cytotoxicity) and high CDC (complement-dependent cytotoxicity) effector functions [1].

The sequence of Anti-β-Gal-mIgG2a InvivoFit™ is 100% murine (constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. This feature allows for reduced immunogenicity and risks of fatal hypersensitivity reactions upon repeated mAb injections into mice [2]. 

Anti-β-Gal-mIgG2a InvivoFit™ is provided in the InvivoFit™ grade, suitable for in vivo studies. All InvivoFit™ products are handled in a clean room, filter-sterilized, and tested for bacterial contaminants. Additionally, this grade guarantees low levels of endotoxins (<1 EU/ml). The buffer formulation is specifically adapted for in vivo studies.

Key features:

• Targets E. coli β-galactosidase antigen 
• Sequence is 100% murine
• Murine IgG2 isotype (constant region)
• Very high ADCP, high ADCC, and high CDC
• Free from non-relevant mAbs found in hybridoma-based productions
• Produced in animal-free facilities and defined media
• Low aggregation < 5%
• InvivoFit™ grade

References:

1. Nimmerjahn F. et al., 2005. FcgammaRIV: a novel FcR with distinct IgG subclass specificity. Immunity. 2005 Jul;23(1):41-51.

Specifications

Target: E. coli β-galactosidase (β-Gal)

Specificity: no cross-reaction with murine proteins

Clone: T9C6

Source: CHO cells

Isotype: Murine IgG2a, kappa

Formulation of Anti-β-Gal-mIgG2a InvivoFit™: Lyophilized from 0.2 µm filtered solution in a sodium phosphate buffer with 5% saccharose

Application: Negative control for murine IgG2a antibodies

Quality control:

• The absence of binding was confirmed by flow cytometry.
• The complete sequence of this antibody has been verified.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.
• The endotoxin level is <1 EU/mg (determined by a LAL assay).

Contents

Anti-β-Gal-mIgG2a InvivoFit™ is filter-sterilized (0.2 µm), endotoxin-free, azide-free, and lyophilized.  

This product is available in three pack sizes:

• bgal-mab10-1​: 1 mg
• bgal-mab10-10​: 10 mg
• bgal-mab10-50​: 50 mg (5 x 10 mg)

 The product is shipped at room temperature.

Store lyophilized antibody at -20 °C.

Lyophilized product is stable for at least 1 year.

InvivoFit™

InvivoFit™ is a high-quality standard specifically adapted for in vivo studies. InvivoFit™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and a TLR2 and TLR4 reporter assay.

Citations